igiciro gito cya Alkaline ProteinaseK CAS 39450-01-6
Twiyemeje gutanga ibicuruzwa byiza kandi bishimishije, hamwe n'ibisubizo byiza cyane, ndetse no gutanga byihuse ku giciro gito cya Alkaline mu ruganda.ProteinaseK CAS 39450-01-6Twifuje cyane gukorana n'abaguzi hirya no hino ku isi. Twizera ko tuzabahaza. Twakira neza abaguzi bakajya mu kigo cyacu bakagura ibicuruzwa byacu.
Twiyemeje gutanga ibicuruzwa byiza kandi bishimishije, kandi bitanga serivisi nziza kandi byihuse kuProteyine ya Alkaline yo mu Bushinwa na 39450-01-6Kubera ubwiza bwiza n'ibiciro biri ku rugero rwiza, ibicuruzwa byacu byoherejwe mu bihugu birenga 10 n'uturere. Twiteguye gukorana n'abakiriya bose bo mu gihugu no mu mahanga. Byongeye kandi, kunyurwa n'abakiriya ni cyo kintu duharanira iteka ryose.
| Ikinyobwa No.1 | |
| Igikorwa cya poroteyine kiri hejuru ya 800U/mL, igipimo ni 20mg/mL, nta bisigazwa bya Nickase | |
| Ifu ya No.2 | |
| Isura | Ifu y'umweru ya lyophilized |
| Ubuziranenge bwa electrophoretic | ≥95% |
| Igikorwa cya enzyme | ≥30U/mg |
| Ibisigazwa bya aside nucleic | Ibitagaragara |
| Ibisigazwa bya Deoxyribonuclease | Ibitagaragara |
| Ibisigazwa bya Ribonuclease | Ibitagaragara |
| Nimero y'igare | G1205-10ML |
| Ububiko | Iyo bibitswe kuri -20 °C, ibi bicuruzwa bigumana ibikorwa byibuze umwaka umwe, bikajyanwa mu ifu y'urubura. |
Poroteyine Kni serine protease ikomoka mu muryango wa subtilisin ifite imikorere myiza ya enzymatic hamwe n’imiterere yagutse ya substrate, ikaba igabanya cyane imigozi ya ester na peptides zegeranye na C-terminus ya aside amine hydrophobic, aside amine irimo sulfur, hamwe na aside amine aromatic bonds, zikunze gukoreshwa mu kwangiza poroteyine kugira ngo zikore peptide ngufi. Proteinase K ifite imiterere ya catalytic triad isanzwe Asp39-His69-Ser224 yihariye kuri serine proteases kandi ifite ahantu habiri ho gufata Ca2+ hafi y’ikigo gikora kugira ngo yongere ubushobozi bwayo bwo kugumana imikorere myiza ya enzymatic mu bihe bitandukanye.
1. Imashini yo gusuzuma imiterere y'uturemangingo.
2. Ibikoresho byo gukuramo RNA na ADN.
3. Kuramo ibice bitari poroteyine mu ngingo no kwangiza imyanda ya poroteyine (nk'itegurwa ry'inkingo za ADN na heparin).
4. Gutegura ADN ya Chromosomal kugira ngo ikoreshwe mu gupima electrophoresis y'imitsi.
5. Gukuraho ibidukikije mu burengerazuba.
6. Guteza imbere no gukora ibikoresho bya enzyme glycated albumin reagents mu rwego rwo gusuzuma indwara zakorewe muri vitro.
1. 30mg/icupa 1g/icupa cyangwa 100g/icupa
2. 1kg/isakoshi ya foil
Proteyine K ni proteyine ya serine ihamye ifite ubushobozi bwagutse bwo gukora ibintu bitandukanye. Yangiza poroteyine nyinshi mu miterere yayo ndetse no mu gihe hari isuku. Ntikoreshwa na iyoni z'icyuma, ibintu bitera uburyaryate (urugero, EDTA), ibitera ubwiza bya sulfhydryl, cyangwa na tripsin cyangwa chymotrypsin. Irahamye ku rugero rwa pH (4–12.5), hamwe n'imikorere myiza kuri pH 6.5–9.5. Ibikorwa bishobora gushishikarizwa no kongeramo ibintu bihindura imiterere y'umubiri (SDS na Urea). Guhindura imiterere y'umubiri byihuse biba ku bushyuhe buri hejuru ya 70°C. Guhindura imiterere y'umubiri byihuse biba kuri pH ya alkaline. Ariko, Proteyine K ntabwo ihinduka burundu binyuze mu guhindura imiterere y'umubiri. Uduce tumwe na tumwe twa enzyme dukomeza gukora neza, ndetse na nyuma yo guhindura imiterere y'umubiri byihuse. Proteyine K ikunze gukoreshwa mu buryo bwa molekile mu gusya poroteyine zidakenewe, nka nucleases mu gutegura ADN cyangwa RNA bivuye mu tunyangingo duto, uturemangingo twakuriye, n'ibimera. Ubusanzwe iyi enzyme ikoreshwa kuri 50–200 ug/ml mu gutegura aside nucleic kuri pH 7.5–8.0 na 37~55oC. Igihe cyo gutera intanga kiratandukanye kuva ku minota 30 kugeza ku masaha 18.
Protease ya serine ihamye kandi ikora neza cyane; ikoreshwa mu gutandukanya poroteyine na aside nucleic. Proteinase K ikoreshwa mu gutunganya RNA na ADN mu bice by'umubiri cyangwa mu turemangingo.
Twiyemeje gutanga ibicuruzwa byiza kandi bishimishije, kandi bitanga serivisi nziza kandi byihuse ku giciro gito cya Alkaline ProteinaseK mu ruganda.CAS39450-01-6Twifuje cyane gukorana n'abaguzi hirya no hino ku isi. Twizera ko tuzabahaza. Twakira neza abaguzi bakajya mu kigo cyacu bakagura ibicuruzwa byacu.
igiciro gito cy'urugandaProteyine ya Alkaline yo mu Bushinwa na 39450-01-6Kubera ubwiza bwiza n'ibiciro biri ku rugero rwiza, ibicuruzwa byacu byoherejwe mu bihugu birenga 10 n'uturere. Twiteguye gukorana n'abakiriya bose bo mu gihugu no mu mahanga. Byongeye kandi, kunyurwa n'abakiriya ni cyo kintu duharanira iteka ryose.
