uruganda igiciro gito Alkaline ProteinaseK CAS 39450-01-6

Twiyemeje gutanga igipimo cyo gupiganwa, ibicuruzwa bidasanzwe nibisubizo byujuje ubuziranenge, cyane nko gutanga byihuse ku ruganda ruto AlkalineProteinaseK CAS 39450-01-6, Twifuzaga tubikuye ku mutima gufatanya n'abaguzi ahantu hose ku isi. Turizera ko tuzaguhaza. Twishimiye kandi abaguzi kujya mumuryango wacu no kugura ibicuruzwa byacu.
Twiyemeje gutanga igipimo cyo gupiganwa, ibicuruzwa bidasanzwe nibisubizo byujuje ubuziranenge, cyane nko gutanga byihuseUbushinwa Alkaline ProteinaseK na 39450-01-6, Kubera ubuziranenge bwiza nibiciro byumvikana, ibicuruzwa byacu byoherejwe mubihugu n'uturere birenga 10. Dutegereje gufatanya nabakiriya bose baturutse mu gihugu ndetse no hanze yarwo. Byongeye kandi, kunyurwa kwabakiriya nibyo dukurikirana ubuziraherezo.

Proteinase K.ni poroteyine ya serine yumuryango wa subtilisin ifite ibikorwa byimikorere myiza kandi ifite insimburangingo yagutse, ikunda kubora ester bonds na peptide iherekejwe na C-terminus ya acide hydrophobique amino acide, sulfure irimo aside amine acide, hamwe na aromatic amino acide, aribyo bikunze gukoreshwa mu gutesha agaciro poroteyine kugirango zitange peptide ngufi. Proteinase K ifite ibiranga ubutatu busanzwe bwa catalitiki Asp39-His69-Ser224 yihariye proteine ​​ya serine kandi ifite ibibanza bibiri bya Ca2 + bihuza ikigo gikora kugirango byongere ituze kandi bikomeze ibikorwa byimisemburo myinshi mubihe byinshi.

1. Igikoresho cyo gusuzuma indwara.
2. Ibikoresho byo gukuramo RNA na ADN.
3. Kuramo ibice bitari poroteyine mu ngingo no gutesha agaciro umwanda wa poroteyine (nko gutegura inkingo za ADN na heparin).
4. Gutegura ADN ya Chromosomal kuri Pulse Electrophoresis.
5. Guhindura iburengerazuba.
6. Gutezimbere no gutanga umusaruro wa enzymatique glycated albumin reagents mubijyanye no gusuzuma vitro.

1. 30mg / icupa 1g / icupa cyangwa 100g / icupa
2. 1kgs / igikapu

Proteinase K ni proteine ​​ihamye hamwe na substrate yagutse. Itesha agaciro poroteyine nyinshi muri kavukire ndetse no imbere yimyenda. Ntabwo ikoreshwa na ioni yicyuma, imiti ya chelating (urugero, EDTA), sulfhydryl reagents, cyangwa na tripine cyangwa chymotrypsin inhibitor. Irahagaze hejuru ya pH yagutse (4–12.5), hamwe nibikorwa byiza kuri pH 6.5-9.5. Igikorwa kirashobora gushishikarizwa no kongeramo ibintu (SDS na Urea). Gutandukana vuba kwa enzyme bibaho ku bushyuhe buri hejuru ya 70 ° C. Autolysis ya enzyme ibaho cyane kuri alkaline pH. Nyamara, Proteinase K ntabwo idakorwa rwose na autolysis. Ibice bimwe bya enzyme bikomeza kugumana ibikorwa bya proteolyique byuzuye, nubwo nyuma ya autolysis yagutse. Proteinase K ikoreshwa cyane mubinyabuzima bya molekuline ikoreshwa mu gusya poroteyine zidakenewe, nka nuclease muri ADN cyangwa imyiteguro ya RNA ituruka kuri mikorobe, ingirabuzimafatizo, n'ibimera. Enzyme isanzwe ikoreshwa kuri 50–200 ug / ml mugutegura aside nucleic kuri pH 7.5-8.0 na 37 ~ 55oC. Ibihe byubushakashatsi biratandukanye kuva muminota 30 kugeza kumasaha 18.

Poroteyine ihamye kandi ikora cyane; ikoreshwa muri poroteyine na acide nucleic aside.Proteinase K ikoreshwa mugusukura RNA na ADN biva mumyenda cyangwa imirongo ya selile.

Twiyemeje gutanga igipimo cyo gupiganwa, ibicuruzwa bidasanzwe nibisubizo byujuje ubuziranenge, cyane nko gutanga byihuse kubiciro biciriritse Alkaline ProteinaseKCAS39450-01-6, Twifuzaga tubikuye ku mutima gufatanya n'abaguzi ahantu hose ku isi. Turizera ko tuzaguhaza. Twishimiye kandi abaguzi kujya mumuryango wacu no kugura ibicuruzwa byacu.
uruganda igiciro gitoUbushinwa Alkaline ProteinaseK na 39450-01-6, Kubera ubuziranenge bwiza nibiciro byumvikana, ibicuruzwa byacu byoherejwe mubihugu n'uturere birenga 10. Dutegereje gufatanya nabakiriya bose baturutse mu gihugu ndetse no hanze yarwo. Byongeye kandi, kunyurwa kwabakiriya nibyo dukurikirana ubuziraherezo.