Icyamamare Cyinshi Kugurisha Proteinase K CAS 39450-01-6 Ubwiza Bwizewe
Twibwira icyo abakiriya batekereza, byihutirwa byihutirwa gukora imbere yinyungu zumwanya wumukiriya wihame shingiro, kwemerera ubuziranenge bwiza cyane, kugabanya ibiciro byo gutunganya, kwishyurwa birumvikana, gutsindira abakiriya bashya nababanjirije inkunga no kubyemeza Icyamamare Cyinshi KugurishaProteinase K CAS 39450-01-6Ubwishingizi bufite ireme, Nka nzobere kabuhariwe muri uru rwego, twiyemeje gukemura ikibazo icyo ari cyo cyose cyo kurinda ubushyuhe bukabije ku bakoresha.
Twibwira icyo abakiriya batekereza, byihutirwa byihutirwa gukora imbere yinyungu zumwanya wumukiriya wihame shingiro, kwemerera ubuziranenge bwiza cyane, kugabanya ibiciro byo gutunganya, kwishyurwa birumvikana, gutsindira abakiriya bashya nababanjirije inkunga no kubyemezaUbushinwa Proteinase K na CAS 39450-01-6, Hariho ibikoresho bigezweho byo gutunganya no gutunganya hamwe nabakozi bafite ubuhanga kugirango barebe ibintu bifite ubuziranenge. Twabonye serivisi nziza mbere yo kugurisha, kugurisha, nyuma yo kugurisha kugirango tumenye abakiriya bashobora kwiringira gutanga ibicuruzwa. Kugeza ubu ibisubizo byacu bigenda byihuta kandi bizwi cyane muri Amerika yepfo, Aziya yuburasirazuba, uburasirazuba bwo hagati, Afrika, nibindi.
| No.1 amazi | |
| Poroteyine ibikorwa byihariye> 800U / mL, kwibanda ni 20mg / mL, nta bisigara bya Nickase | |
| Ifu ya 2 | |
| Kugaragara | Ifu yera ya lyofilize |
| Amashanyarazi meza | ≥95% |
| Igikorwa cya Enzyme | ≥30U / mg |
| Ibisigisigi bya aside nucleique | Ibitaboneka |
| Deoxyribonuclease ibisigisigi | Ibitaboneka |
| Ibisigisigi | Ibitaboneka |
| Ikarita No. | G1205-10ML |
| Ububiko | Iyo bibitswe kuri 20 ° C, ibicuruzwa bigumana ibikorwa byibura imyaka 1, gutwara imifuka ya ice. |
Proteinase K.ni poroteyine ya serine yumuryango wa subtilisin ifite ibikorwa byimikorere myiza kandi ifite insimburangingo yagutse, ikunda kubora ester bonds na peptide iherekejwe na C-terminus ya acide hydrophobique amino acide, sulfure irimo aside amine acide, hamwe na aromatic amino acide, aribyo bikunze gukoreshwa mu gutesha agaciro poroteyine kugirango zitange peptide ngufi.Proteinase K.ifite ibiranga ubutatu busanzwe bwa catalitiki Asp39-His69-Ser224 yihariye proteine ya serine kandi ifite ibibanza bibiri bya Ca2 + bihuza ikigo gikora kugirango yongere ituze kandi ikomeze ibikorwa byimisemburo myinshi mubihe byinshi.
1. Igikoresho cyo gusuzuma indwara.
2. Ibikoresho byo gukuramo RNA na ADN.
3. Kuramo ibice bitari poroteyine mu ngingo no gutesha agaciro umwanda wa poroteyine (nko gutegura inkingo za ADN na heparin).
4. Gutegura ADN ya Chromosomal kuri Pulse Electrophoresis.
5. Guhindura iburengerazuba.
6. Gutezimbere no gutanga umusaruro wa enzymatique glycated albumin reagents mubijyanye no gusuzuma vitro.
1. 30mg / icupa 1g / icupa cyangwa 100g / icupa
2. 1kgs / igikapu
Proteinase K ni proteine ihamye hamwe na substrate yagutse. Itesha agaciro poroteyine nyinshi muri kavukire ndetse no imbere yimyenda. Ntabwo ikoreshwa na ioni yicyuma, imiti ya chelating (urugero, EDTA), sulfhydryl reagents, cyangwa na tripine cyangwa chymotrypsin inhibitor. Irahagaze hejuru ya pH yagutse (4–12.5), hamwe nibikorwa byiza kuri pH 6.5-9.5. Igikorwa kirashobora gushishikarizwa no kongeramo ibintu (SDS na Urea). Gutandukana vuba kwa enzyme bibaho ku bushyuhe buri hejuru ya 70 ° C. Autolysis ya enzyme ibaho cyane kuri alkaline pH. Nyamara, Proteinase K ntabwo idakorwa rwose na autolysis. Ibice bimwe bya enzyme bikomeza kugumana ibikorwa bya proteolyique byuzuye, nubwo nyuma ya autolysis yagutse. Proteinase K ikoreshwa cyane mubinyabuzima bya molekuline ikoreshwa mu gusya poroteyine zidakenewe, nka nuclease muri ADN cyangwa imyiteguro ya RNA ituruka kuri mikorobe, ingirabuzimafatizo, n'ibimera. Enzyme isanzwe ikoreshwa kuri 50–200 ug / ml mugutegura aside nucleic kuri pH 7.5-8.0 na 37 ~ 55oC. Ibihe byubushakashatsi biratandukanye kuva muminota 30 kugeza kumasaha 18.
Poroteyine ihamye kandi ikora cyane; ikoreshwa muri poroteyine na acide nucleic aside.Proteinase K ikoreshwa mugusukura RNA na ADN biva mumyenda cyangwa imirongo ya selile.
Twibwira icyo abakiriya batekereza, byihutirwa byihutirwa gukora imbere yinyungu zumwanya wumukiriya wihame shingiro, kwemerera ubuziranenge bwiza cyane, kugabanya ibiciro byo gutunganya, kwishyurwa birumvikana, gutsindira abakiriya bashya nababanjirije inkunga no kubyemeza Icyamamare Cyinshi Kugurisha Proteinase K CAS39450-01-6Ubwishingizi bufite ireme, Nka nzobere kabuhariwe muri uru rwego, twiyemeje gukemura ikibazo icyo ari cyo cyose cyo kurinda ubushyuhe bukabije ku bakoresha.
Icyubahiro cyinshiUbushinwa Proteinase K na CAS 39450-01-6, Hariho ibikoresho bigezweho byo gutunganya no gutunganya hamwe nabakozi bafite ubuhanga kugirango barebe ibintu bifite ubuziranenge. Twabonye serivisi nziza mbere yo kugurisha, kugurisha, nyuma yo kugurisha kugirango tumenye abakiriya bashobora kwiringira gutanga ibicuruzwa. Kugeza ubu ibisubizo byacu bigenda byihuta kandi bizwi cyane muri Amerika yepfo, Aziya yuburasirazuba, uburasirazuba bwo hagati, Afrika, nibindi.
