Igishushanyo mbonera cy'umwuga cya Proteyine K nziza cyane CAS No. 39450-01-6 ku giciro cyiza
Ubusanzwe dukora nk'abakozi bafatika kugira ngo tubahe umusaruro mwiza cyane hamwe n'igiciro cyiza cyo kugurisha ku giciro cyiza cyane cy'ubuhanga mu gushushanya.Poroteyine K CAS No. 39450-01-6hamwe n'igiciro cyiza, Kugira ngo ubone amakuru n'ibindi bisobanuro birambuye, tuvugishe vuba bishoboka!
Ubusanzwe dukora nk'abakozi bafatika twizeza ko tuzabaha ibyiza cyane hamwe n'igiciro cyiza cyo kugurisha.Ubushinwa bwa Tritirachium Album Limber na Molecular Biology, Igenzura ry’ubuziranenge rishyirwa mu bikorwa muri buri sano ry’uburyo bwose bwo gukora. Twiringiye cyane ko tuzashyiraho ubufatanye bwiza kandi bufitiye akamaro. Dushingiye ku bisubizo byiza na serivisi nziza mbere yo kugurisha/kugurisha, ni igitekerezo cyacu, bamwe mu bakiriya bamaze imyaka irenga 5 bakorana natwe.
| Ikinyobwa No.1 | |
| Igikorwa cya poroteyine kiri hejuru ya 800U/mL, igipimo ni 20mg/mL, nta bisigazwa bya Nickase | |
| Ifu ya No.2 | |
| Isura | Ifu y'umweru ya lyophilized |
| Ubuziranenge bwa electrophoretic | ≥95% |
| Igikorwa cya enzyme | ≥30U/mg |
| Ibisigazwa bya aside nucleic | Ibitagaragara |
| Ibisigazwa bya Deoxyribonuclease | Ibitagaragara |
| Ibisigazwa bya Ribonuclease | Ibitagaragara |
| Nimero y'igare | G1205-10ML |
| Ububiko | Iyo bibitswe kuri -20 °C, ibi bicuruzwa bigumana ibikorwa byibuze umwaka umwe, bikajyanwa mu ifu y'urubura. |
Poroteyine Kni serine protease iri mu muryango wa subtilisin ifite imikorere myiza ya enzyme hamwe n’imiterere yagutse ya substrate, ikaba igabanya cyane imigozi ya ester na peptides zegeranye na C-terminus ya aside amine hydrophobic, aside amine irimo sulfur, hamwe na aside amine aromatic bonds, akenshi zikoreshwa mu kwangiza poroteyine kugira ngo zikore peptide ngufi.Poroteyine Kifite imiterere ya Asp39-His69-Ser224 isanzwe igizwe na serine proteases kandi ifite ahantu habiri ho gufata Ca2+ hafi y’aho ikorera kugira ngo yongere ubushobozi bwayo bwo kugumana imbaraga nyinshi no gukomeza gukora enzyme nyinshi mu bihe bitandukanye.
1. Imashini yo gusuzuma imiterere y'uturemangingo.
2. Ibikoresho byo gukuramo RNA na ADN.
3. Kuramo ibice bitari poroteyine mu ngingo no kwangiza imyanda ya poroteyine (nk'itegurwa ry'inkingo za ADN na heparin).
4. Gutegura ADN ya Chromosomal kugira ngo ikoreshwe mu gupima electrophoresis y'imitsi.
5. Gukuraho ibidukikije mu burengerazuba.
6. Guteza imbere no gukora ibikoresho bya enzyme glycated albumin reagents mu rwego rwo gusuzuma indwara zakorewe muri vitro.
1. 30mg/icupa 1g/icupa cyangwa 100g/icupa
2. 1kg/isakoshi ya foil
Proteyine K ni proteyine ya serine ihamye ifite ubushobozi bwagutse bwo gukora ibintu bitandukanye. Yangiza poroteyine nyinshi mu miterere yayo ndetse no mu gihe hari isuku. Ntikoreshwa na iyoni z'icyuma, ibintu bitera uburyaryate (urugero, EDTA), ibitera ubwiza bya sulfhydryl, cyangwa na tripsin cyangwa chymotrypsin. Irahamye ku rugero rwa pH (4–12.5), hamwe n'imikorere myiza kuri pH 6.5–9.5. Ibikorwa bishobora gushishikarizwa no kongeramo ibintu bihindura imiterere y'umubiri (SDS na Urea). Guhindura imiterere y'umubiri byihuse biba ku bushyuhe buri hejuru ya 70°C. Guhindura imiterere y'umubiri byihuse biba kuri pH ya alkaline. Ariko, Proteyine K ntabwo ihinduka burundu binyuze mu guhindura imiterere y'umubiri. Uduce tumwe na tumwe twa enzyme dukomeza gukora neza, ndetse na nyuma yo guhindura imiterere y'umubiri byihuse. Proteyine K ikunze gukoreshwa mu buryo bwa molekile mu gusya poroteyine zidakenewe, nka nucleases mu gutegura ADN cyangwa RNA bivuye mu tunyangingo duto, uturemangingo twakuriye, n'ibimera. Ubusanzwe iyi enzyme ikoreshwa kuri 50–200 ug/ml mu gutegura aside nucleic kuri pH 7.5–8.0 na 37~55oC. Igihe cyo gutera intanga kiratandukanye kuva ku minota 30 kugeza ku masaha 18.
Protease ya serine ihamye kandi ikora neza cyane; ikoreshwa mu gutandukanya poroteyine na aside nucleic. Proteinase K ikoreshwa mu gutunganya RNA na ADN mu bice by'umubiri cyangwa mu turemangingo.
Ubusanzwe dukora nk'abakozi bafatika twizeza ko tuzabaha ibyiza cyane hamwe n'igiciro cyiza cyo kugurisha cya Proteinase K y'Umwuga ifite ubuziranenge bwo hejuru CAS No. 39450-01-6 hamwe n'igiciro cyiza, Kubindi bisobanuro n'amakuru, twandikire vuba bishoboka!
Igishushanyo mbonera cy'umwugaUbushinwa bwa Tritirachium Album Limber na Molecular Biology, Igenzura ry’ubuziranenge rishyirwa mu bikorwa muri buri sano ry’uburyo bwose bwo gukora. Twiringiye cyane ko tuzashyiraho ubufatanye bwiza kandi bufitiye akamaro. Dushingiye ku bisubizo byiza na serivisi nziza mbere yo kugurisha/kugurisha, ni igitekerezo cyacu, bamwe mu bakiriya bamaze imyaka irenga 5 bakorana natwe.
